Implications of multiple freeze-thawing on respiratory samples for culture-independent analyses
نویسندگان
چکیده
منابع مشابه
Implications of multiple freeze-thawing on respiratory samples for culture-independent analyses✩
BACKGROUND Best practice when performing culture-independent microbiological analysis of sputum samples involves their rapid freezing and storage at -80°C. However, accessing biobanked collections can mean that material has been passed through repeated freeze-thaw cycles. The aim of this study was to determine the impact of these cycles on microbial community profiles. METHODS Sputum was coll...
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ژورنال
عنوان ژورنال: Journal of Cystic Fibrosis
سال: 2015
ISSN: 1569-1993
DOI: 10.1016/j.jcf.2014.10.004